Expression, purification and functional characterization of recombinant hypervariable region (HVR) of Chikungunya virus nsP3 protein
نویسندگان
چکیده
منابع مشابه
Prokaryotic Expression, Purification, and Polyclonal Antibody Production of a Truncated Recombinant Rabies Virus L Protein
Background: Rabies virus (RABV) is a deadly neurotropic virus that causes the disease of rabies in humans and animals. L protein is one of the large structural protein of rabies virus, which displays multiple enzymatic activities, and is required for viral transcription and replication. Objectives: A truncated L protein of Rabies virus is being cloned, expressed and purified to produce relevant...
متن کاملCloning and Codon-optimized Expression of Structural Protein Hypervariable Region of VP2 from Infectious Bursal Disease Virus
Infectious bursal disease virus (IBDV) is the causative agent of Gumboro disease, an infectious disease of global economic importance in poultry. Structural protein VP2 of IBDV is the most frequently studied protein due to its significant roles in virus attachment, protective immunity, and serotype specificity. The objective of the present study was to improve the expression of hypervariable re...
متن کاملCloning, expression and purification of Chikungunya virus E2 recombinant protein in E.coli
Background Chikungunya virus infection is an emerging arbovirus disease. While many infected individuals recover after primary illness, others suffer from persistent debilitating arthralgia that can last for months to years. Currently, there are no immune correlates, vaccines, therapies or robust diagnostics that can affectively prevent, treat or diagnose Chikungunya. Therefore, this study focu...
متن کاملExpression and Purification of Recombinant Outer Surface Protein D of Borrelia burgdorferi
To carry out the immunological experiments on the serum of Multiple Sclerosis (MS) patients, based on a correlation between Borrelia burgdorferi infection and contracting MS autoimmune disease the outer surface protein D (OspD) of the bacterium was expressed and purified. A clone containing the OspD gene in pET11a expression vector under the control of T7 promoter was transformed to the bacteri...
متن کاملStimulation of dendritic cell functional maturation by capsid protein from chikungunya virus
Objective(s): Chikungunya virus (ChikV) infection is characterized by persistent infection in joints and lymphoid organs. The ChikV Capsid protein plays an important role in regulating virus replication. In this study, we hypothesized that capsid protein may stimulate dendritic cell (DC) activation and maturation and trigger an inflammatory response in mice. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: 3 Biotech
سال: 2019
ISSN: 2190-572X,2190-5738
DOI: 10.1007/s13205-019-1759-8